Human immune-monitoring core

The human immune-monitoring core of the Physiopathology Center of Toulouse Purpan specializes in the biobanking of human immune cells and the development of standardized techniques for the evaluation of individual immunological profiles and functional responses of human immune cells in the context of clinical studies (infection, autoimmunity, inflammation). Specifically, we develop and distribute a collection of frozen PBMC from more than 250 healthy subjects. In partnership with the laboratories of the center, we perform rotations of about 8 months on specific projects with a focus on technical set-ups and regulatory affairs related to human research.

Our team

Manager: Raphaëlle ROMIEU-MOUREZ


Services

Healthy subject PBMC biobanking
  • PBMC are isolated from buffy coats provided by the “Établissement Français du Sang” (EFS) at the Purpan hospital in Toulouse, France
  • The PBMC bank is from adult volunteers donating their blood for a therapeutic use and who are recruited in the greater Toulouse – Occitania region. Donors reported feeling well and were evaluated in good general health by a doctor at the EFS. Each blood donation was monitored for the absence of permanent contraindication for blood donation (viral hepatitis, syphilis, HIV or HTLV infection)
  • PBMC are characterized for donor gender, age, blood type, HLA-A2 and HLA-B8 expression, and for some donors CMV serology
  • 110 new donors are added annually to the biobank in order to reach a constant availability of 250 donors
  • The core facility provides frozen PBMC vials, each containing 20 million PBMC
  • At least 1 vial from each donor is thawed and analyzed by flow cytometry. In addition, vials from 10 donors are sided apart for the annual quality controls that consist of the measurement of CD4+ T and CD8+ T cell numbers and viability and the production of interferon by memory CD8+ T cells.
  • PBMC request form (only for CPTP members) : r- PBMC request send form to contact
Development and standardization of immune assays on human cells

  • This activity is carried out during 8-months rotations in laboratories involved in human immune-monitoring studies
  • We provide support for the definition of project-specific immune assays, in addition we evaluate their inter-experimental variability across experimenters and over time

Example of projects : ex vivo ELISPOT assays for the measurement of memory T cell response specific for the hepatitis E virus, definition and testing of multiparametric flow cytometric profiles, isolation of rare Treg cell population circulating toward the thymus, handling of low-volume blood samples such as pediatric samples

 

Human research regulatory affairs

We act as a liaison with several institutions’ departments of regulatory affairs to ensure that the center complies with the ethical and regulatory requirements for human research. Examples: writing of patient information sheets, informed consent forms, or administrative documents related to collections of human biological samples.

Useful links to understand the rules of clinical research in France:

Equipments

AutoMACS Pro cell separator (Miltenyi Biotec)

P1012091 AutoMACS

  • Users trained for a 1st use
  • All buffers provided to users
  • In the L2 lab – F building

 

Booking though the center intranet website

Fees for members of the CPTP : per sample 7 euros (sample ≤ 200 million cells) or 14 euros (sample > 200 million cells)

ELISPOT reader

  • S6 Ultra-V analyzer (Cellular Technology Ltd)
  • In the L2 lab – F building
  • No fees

Other informations


Steering committee
  • Eric Champagne
  • Loïc Dupré
  • Nicolas Fazilleau
  • Sylvie Guerder
  • Jean-Charles Guéry
  • Roland Liblau
  • Lucette Pelletier
  • Joost van Meerwijk
Publications
Inserm

Inserm

CNRS

CNRS

UT3

UT3