Human immuno-monitoring core

The human immune-monitoring core of the Centre de Physiopathologie de Toulouse Purpan (CPTP) specializes in the biobanking of human immune cells and in the development of standardized techniques for the evaluation of immunological profiles and functional responses of human immune populations in the context of clinical studies (infection, autoimmunity, inflammation). Specifically, we develop and distribute a collection of frozen PBMC from more than 250 healthy subjects. In partnership with the laboratories of the center, we perform rotations of about 8 months on specific projects in human immunological monitoring, with a focus on technical set-ups and regulatory affairs related to human research.

Our team

Leader : Raphaëlle Romieu-Mourez


Prestations

Healthy subject PBMC biobanking

  • PBMC are isolated from buffy coats provided by the “Établissement Français du Sang” (EFS) at the Purpan hospital in Toulouse, France;
  • the bank is from adult volunteers donating their blood for a therapeutic use and who are recruited in the greater Toulouse – Occitania region. Donors reported feeling well and were evaluated in good general health by a doctor at the EFS. Each blood donation was monitored for the absence of permanent contraindication for blood donation (viral hepatitis, syphilis, HIV or HTLV infection);
  • PBMC are characterized for donor gender, age, blood type, HLA-A2 and HLA-B8 expression, and for some donors CMV serology;
  • approximately 110 new donors are added annually to the biobank in order to reach a constant availability of 250 donors;
  • the core facility provides frozen PBMC vials, each containing 20 million PBMC;
  • at least 1 vial from each donor is thawed and analyzed by flow cytometry. In addition, vials from 10 donors are sided apart for the annual quality controls that consist of the measurement of CD4+ T and CD8+ T cell numbers and viability and the production of interferon by memory CD8+ T cells;

 

 

Protocols

Healthy subject PBMC biobanking

  • this activity is carried out during 8-months rotations in laboratories involved in human immune-monitoring studies;
  • we provide support for the definition of project-specific designs and the testing of immune assays such as ex vivo ELISPOT assays for the measurement of memory T cell response, the definition and testing of multiparametric flow cytometric profiles, the testing of isolation procedures for immune cell populations. Setting-ups also evaluate the inter-experimental variability of immune assays by our staff and the recipient’s laboratory members.
Development and/or standardization of immune assays on human cells

  • this activity is carried out during 8-months rotations in laboratories involved in human immune-monitoring studies;
  • we provide support for the definition of project-specific designs and the testing of immune assays such as ex vivo ELISPOT assays for the measurement of memory T cell response, the definition and testing of multiparametric flow cytometric profiles, the testing of isolation procedures for immune cell populations. Setting-ups also evaluate the inter-experimental variability of immune assays by our staff and the recipient’s laboratory members.

Equipment

AutoMACS Pro cell separator (Miltenyi Biotec)

  • P1012091 AutoMACSlocalised in L2 biosecurity level
  • formation assurée pour une première utilisation
  • all buffers are provided to users

 

Booking though the center intranet website

Fees for members of the CPTP : per sample 7 euros (less than 200 million cells) or 14 euros (more than 200 million cells)

ELISPOT reader

  • S6 Ultra-V analyzer (Cellular Technology Ltd)
  • no fees

Contact & booking


The technical platform is accessible in self-service after a compulsory training, and on reservation (electronic planning).

Other informations


Steering committee
  • Eric Champagne,
  • Loïc Dupré,
  • Nicolas Fazilleau,
  • Sylvie Guerder,
  • Jean-Charles Guéry,
  • Roland Liblau,
  • Lucette Pelletier,
  • Joost van Meerwijk,
  • Salvatore Valitutti.
Publications
Inserm

Inserm

CNRS

CNRS

UT3

UT3