Imaging cell core

The cellular imaging facility of the Physiopathology Center of Toulouse Purpan offers a wide array of biophotonic approaches, allowing to visualize biological events from the nanometric to the organ scale. Microscopes are available for scientists from the CPTP but also from outside the institute (public or private domain). Thanks to the connection of the facility with TRI (Toulouse Réseau Imagerie), the facility is ISO9001 V2015 and NFX 50-900 v2016 certified, in order to ensure a continuous improvement and customer satisfaction.

Our team

Head : Sophie Allart


Service organization

For every new user, we organize a first meeting to help to choose the best protocol and to decide which microscope to use.

The facility offers two types of services:

  • Training: A training provided by the engineers of the facility is mandatory to be granted an access to the microscopes and to book them
  • Expertise: Engineers of the facility can help you for the technical development of your projects and for the analysis of your data.
Training, advice, competence
  • Training to use microscopes
  • Help for implementation or optimization of an experimental protocol
  • Assistance for certain technical approaches
  • Help for image analysis
  • Expertise for private companies
  • Continuing education (INSERM, CNRS, University, private company, Workshop)
Applications
Techniques available on the facility
  • Spectral imaging
  • Time lapse
  • FRET
  • TIRF
  • FRAP/ FLIP/ iFRAP
  • IRM (Interference Reflection Microscopy)
  • Biphoton
  • dSTORM
  • STED
  • Photoactivation
  • uPAINT
  • Image analysis
Object quantification
  • Fluorescence intensity quantification
  • Colocalization
  • Macros for ImageJ
  • 4D analysis with Imaris
  • ImageJ
  • Training for image analysis

Équipements

Microscope confocal Zeiss LSM 510

 

  • Malik Hamaida, Salvatore Valitutti team

    Lasers: 458 nm, 477 nm, 488 nm, 514 nm, 543 nm and 633 nm on an inverted motorized microscope.

  • 8 different dyes can be acquired thanks to the META spectral detector.
  • Objectives:10X plan neofluar (NA: 0.3) ? 20 X plan apo (NA: 0.8),40X plan apo (NA: 1.3), 63X plan apo (NA: 1.4) and 100X plan apo (NA: 1.4)
  • Equipped with temperature and oxygen controllers.
  • FRAP and colocalization modules are available

Booking

Microscope confocal Zeiss LSM 710

Manon Farcé, Salvatore Valitutti team.

  • Lasers: 405 nm, 458 nm, 477 nm, 488 nm, 514 nm, 543 nm et 633 nm on an inverted motorized microscope
    Objectives: 20x plan apo (NA: 0.8) and 63x oil plan apo (NA 1.4).
  •  Equipped with temperature and oxygen controllers.
  • With the spectral detector, autofluorescence can be removed and dyes which are spectrally close can be separated.
  • FRAP and colocalization modules are available

Booking

Microscope confocal Leica SP8- STED 3X

 

  • Elsa Suberbielle, Dunia team, CPTP

    Lasers: 405 nm, 488 nm, 532 nm, 552 nm and  635 nm on an inverted motorized microscope

  • For STED acquisition, excitation lasers are 532 and 635 nm. Depletion laser is 775 nm.
  • Objectives: 20 X Plan Fluotar DIC (NA: 0.8), 63x Oil plan apo DIC (NA 1.4) and a 100X (NA 1.4) for STED acquisitions
  • The system is equipped with 3 high sensitive HyD (QE: 45 % instead of 25 % for PMT) and 2 PMT.
  • Equipped with temperature and oxygen controllers.

Booking

Microscope bi-photon ZEISS LSM 7 MP droit

 

  • Lidia Yshii, Roland Liblau team

    Upright multi photon Zeiss LSM 7MP microscope for cellular and tissular multicolor acquisitions thanks to the 5-channel NDD. The pulsed laser Chameleon Ultra II is adjustable from 690 to 1080 nm.

  • Objectives: dipping 20X Plan-Apo (NA: 1), 40X oil Plan-Apo (NA: 1.4) and 40X water immersion (NA: 1.1).
  • The microscope is fully encaged and regulated for temperature and CO2 to maintain cells in optimal growth conditions.
  • It is also equipped for tissue explant for hours.

Booking

Apotome

  • This microscope can be used as a wide field but it can also remove out of focus fluorescence to allow optical sectioning.
  • It is also possible to perform image tiling with a perfect stitching.
  • Exact positions can be studied for several days with a perfect repositioning.
  • The microscope is fully encaged and regulated for temperature and CO2 to maintain cells in optimal growth conditions.
  •  Objectives: 10X, 20X, 40X and 63X.
  • Fluorescent filters for DAPI, Green, Red and Far Red.

L. Pellerin, Simon Team, UDEAR

Booking

Microscope confocal Spinning Disk- TIRF- FRAP

  • Lasers: 375 nm (for photoactivation), 405 nm, 491 nm, 561 nm and 642 nm.
  • Objectives: 10X Plan Apo (NA 0.75), 20X plan apo (NA 0.75), 40X oil immersion fluor (NA 1.3) suited for UV experiments, 60X oil immersion plan apo (NA 1.4) and 100X oil immersion apochromate (NA 1.49) for TIRF experiments. DIC can be acquired with all the objectives.
  • The CSU-X1 confocal scanner unit allows ultra-fast acquisition times, while limiting photobleaching.
  • ILas² module allows FRAP/FLIP/photoactivation experiments and also azimuthal TIRF and dSTORM.
  • The microscope is fully encaged and regulated for temperature and CO2 to maintain cells in optimal growth conditions.

Booking

Image analysis

 

Three computers are available for image treatment and analysis. You can also use them to perform deconvolution on wide field images. On request, engineers of the facility can also write macros for ImageJ in order to automate your analyses.

Three softwares are available:

  • Metamorph (Universal Imaging)
  • ImageJ: free software mainly for 2D or 2D+ time image analysis. Macros can be written to automate analyses.
  • Imaris: for 3D and 3D+time analysis. Can create surfaces, track cells and generate statistical analyses. Movies can also be created with Imaris.

Contact & Booking


Equipments of the facility are in self-service access after a mandatory training. Booking can be done on the TRI website

The imaging facility of the CPTP is located at the second floor of the F building, Pavillon Lefebvre, within the CHU Purpan.

Other informations


Steering committee
  • Pr S. Valittuti (CPTP-Eq. Valitutti)
  • Dr S. Guerder (CPTP- Eq Guerder)
  • Dr N. Blanchard (CPTP-Eq Blanchard)
  • Dr P. Romagnoli (CPTP-Eq Van Meerwijk)
  • Dr M. Savignac (CPTP -Eq. Guery)
  • Dr F. Briand –Messange (CPTP-Eq. Salles)
  • Dr M. Requena (CPTP-Eq Izopet)
  • Dr R. Lesourne (CPTP-Eq Lesourne)
  • Dr D. Gonzalez-Dunia (CPTP-Eq Dunia/Casper)
  • Dr Saoudi (CPTP-Eq Liblau/Saoudi)
  • Dr C. Leprince (UDEAR)
  • Dr N. Vergnolle (IRSD)
Publications
Occitanie

Occitanie

CNRS

CNRS

Inserm

Inserm

UT3

UT3

TRI-Genotoul

TRI-Genotoul

FRM

FRM